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| Registros recuperados: 148 | |
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| Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema. |
| Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019 |
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| Lagos,Jaime; Alarcón,Pedro; Benadof,Dona; Ulloa,Soledad; Fasce,Rodrigo; Tognarelli,Javier; Aguayo,Carolina; Araya,Pamela; Parra,Bárbara; Olivares,Berta; Hormazábal,Juan Carlos; Fernández,Jorge. |
| Abstract We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Catalase-negative; Catalase gene; KatA. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100177 |
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| Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes. |
| The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018 |
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| Amaral,L.A.; Rossi Júnior,O.D.; Nader Filho,A.; Ferreira,F.L.A.; Barros,L.S.S.. |
| Com o objetivo de verificar o papel da água utilizada durante a produção do leite como via de transmissão de Staphylococcus sp., fez-se a contagem de Staphylococcus coagulase negativa e Staphylococcus aureus nas amostras de água das fontes, dos reservatórios e dos estábulos de 30 propriedades leiteiras situadas na região Nordeste do Estado de São Paulo. As maiores ocorrências de isolamentos (10,0 e 16,6%) e os maiores valores médios (4,3×10(4) e 2,5×10(4)) de contagens desses microrganismos foram encontrados nas amostras de água dos estábulos utilizada na obtenção de leite. Estes resultados são importantes pois evidenciam a possibilidade de contaminação do leite ou dos animais por cepas patogênicas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Água; Staphylococcus sp.; Staphylococcus aureus; Propriedade leiteira. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000500017 |
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| Pelisser,Marcia Regina; Klein,Cátia Silene; Ascoli,Kelen Regina; Zotti,Thaís Regina; Arisi,Ana Carolina Maisonnave. |
| Multiplex PCR was used to investigate the presence of enterotoxins genes (sea, seb, sec, sed and see) and femA gene (specific for Staphylococcus aureus) in coagulase-positive staphylococci (CPS) isolated from cheese and meat products. From 102 CPS isolates, 91 were positive for femA, 10 for sea, 12 for sed and four for see. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Enterotoxins; Multiplex PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100025 |
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| Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.. |
| Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071 |
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| Berberian,Griselda; Firpo,Verónica; Soto,Adriana; Lopez Mañan,Julio; Torroija,Cecilia; Castro,Graciela; Polanuer,Pablo; Espinola,Camilo; Piñeiro,José Luis; Rosanova,María Teresa. |
| OBJECTIVES: The aim of this study was to identify the clinical, radiological, and bacteriological features, risk factors, and outcome of neonates with bone and joint infections. STUDY DESIGN: Observational, retrospective, and analytical study of 77 patients less than 2 months of age, admitted to a tertiary neonatal intensive care unit (NICU) with the diagnosis of bone or joint infection, based on clinical, radiological, and microbiological criteria. RESULTS: Seventy-seven patients with 99 acute osteoarthritis foci in a 16 year period were included in the study. Risk factors for infection could be identified in 69% of the patients. The hip was the most frequent. Staphylococcus aureus was the main isolated microorganism. Twenty-nine infants (38%) had... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Osteoarthritis; Neonate; Staphylococcus aureus. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400018 |
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| Lucero Estrada,Cecilia S.M.; Alcaráz,Lucia E.; Satorres,Sara E.; Manfredi,Eduardo; Velázquez,Lidia del C.. |
| An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Enterotoxigenic strains; Fruit salads; Antimicrobial compounds; Multiplex PCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018 |
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| Paixão,Vilma Almeida; Barros,Tânia Fraga; Mota,Clélia Maria C; Moreira,Tamy Fagundes; Santana,Maria Angélica; Reis,Joice Neves. |
| Respiratory infection is very common in patients suffering from cystic fibrosis (CF). However, the antimicrobial resistance rate of isolates from CF patients is not often documented. In this study, 279 respiratory specimens of 146 patients were prospectively collected from July to December 2006. Microbiological cultures and antimicrobial susceptibility tests of the most frequently isolated bacteria were performed. Sputum and oropharyngeal swabs were processed for culture. During the study period, 50% of the patients harbored Staphylococcus aureus, 35% Pseudomonas aeruginosa, 4.7% Haemophilus influenzae. Methicillin resistant S. aureus (MRSA) were detected in 8 (6%) patients; ESBL and MBL-producing P. aeruginosa were not identified in these patients. The... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cystic fibrosis; Antimicrobial resistance; Pseudomonas aeruginosa; Staphylococcus aureus. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400016 |
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| Silva,Eduardo Caetano Brandão Ferreira da; Antas,Maria das Graças C.; B. Neto,Armando Monteiro; Rabelo,Marcelle Aquino; Melo,Fábio Lopes de; Maciel,Maria Amélia Vieira. |
| Staphylococcus aureus is the main human pathogen that colonizes individuals in general population. The objective of the study was evaluate the epidemiological and sensitivity profile of S. aureus lineage, isolated in health care workers (HCW) of a University Hospital in Pernambuco state, Brazil. Biological samples of hands and nasal cavities were sown in agar sheep blood. Colonies under suspicion of being S. aureus were identified using Gram staining, catalase test and coagulase, mannitol-salty agar fermentation and DNAse agar. The resistance to mupirocin was analyzed through the Kirby Bauer technique. In relation to methicillin and vancomycin the determination was by the minimum inhibitory concentration method (E-test). From the 202 HCW evaluated, 52 were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Colonization; Health staff. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600012 |
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| Ribeiro,Julival; Boyce,John M.; Zancanaro,Pedro Q.. |
| We surveyed patients seen at the emergency room at a tertiary hospital in Brazil from August to November 1997. All patients' (n = 600) anterior nares were cultured for MRSA; the results were confirmed by oxacillin disk diffusion methods and by detection of the mecA gene with PCR. Bacteria were found in 93.3% (560) of the patients and MRSA in 0.7% (n = 4). One patient had community-acquired MRSA. We concluded that MRSA is uncommon among patients visiting the emergency room. The presence of community-acquired MRSA can lead to serious medical and epidemiological issues, although initial clinical presentation may not differ from that of infections with other staphylococci. The empirical use of vancomycin for suspected community-acquired infections is seldom... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Staphylococcus aureus; Emergency room; Brazil. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100009 |
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| Adaleti,Riza; Nakipoglu,Yasar; Ceran,Nurgul; Tasdemir,Cihan; Kaya,Fatma; Tasdemir,Semiha. |
| The incidence of drug-resistant pathogens differs greatly between countries according to differences in the usage of antibiotics. The purpose of this study was to investigate the phenotypic resistance of 321 methicillin resistance Staphylococcus aureus (MRSA) and 195 methicillin susceptible S. aureus (MSSA) in a total of 516 S. aureus strains to macrolide, lincosamide, streptogramin B (MLS B), ketolid, and linezolid. Disk diffusion method was applied to determine MLS B phenotype and susceptibility to different antibiotic agents. It was found that 54.6% of the isolates were resistant to erythromycin (ERSA), 48% to clindamycin, 55% to azithromycin, 58.7% to spiramycin, 34.7% to telithromycin, and 0.4% to quinupristin-dalfopristin, respectively. No strain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Macrolide; Lincosamide; Streptogramin B; Ketolid; Linezolid; MLS B. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100003 |
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| Registros recuperados: 148 | |
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